Ybe Lab

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These are the procedures that we use and links to manuals for things around the lab.

DNA

Purification

• QIAGEN DNA Miniprep Kit
• QIAGEN Gel Extraction Kit

Amplification

• PCR

Mutation

• QuickChange Kit

Other Enzymatic Reactions

• Restriction Enzymes
• NEB Restriction Enzyme Technical Reference Site
• Calf Intestinal Phosphatase
• Ligation
• T4 DNA Ligase

Protein

Gel Stains

• Coomassie Brilliant Blue
• Western Blot (MSWord)
• SYPRO Orange (PDF)
• Stains-All
• Stains-All/Silver Technique for Acidic Proteins (PDF)
• Silver

Proteolysis

• Thrombin (PDF)
• Trypsin

Other

• Acetone Precipitation (PDF)

Chromatography

DNA

Gels

• Agarose

Protein

Gels

• SDS-Tris-Glycine, SDS-Tris-Tricine, and Native-Tris-Glycine Gels, Recipes and Sample Buffers (MSExcel)

Columns

• Sepharose FastFlow Chelating Beads (PDF)
• Regeneration of Chelating Sepharose Beads (MSWord)
• Glutathione Sepharose 4B Beads
• GST Fusion Protein Purification and Bead Renewal (MSWord)
• Heparin
• Poros
• Superdex 75

Cells

• Transformation
• HB101
• DH5a
• Novagen Competent Cells Info for BL21 and Rosetta2 (PDF)

Other

• Protease Inhibitor Info (MSWord)
• Electron Microscopy (MSWord)

Biology Department, Indiana University Bloomington